Kristin Grußmayer


I am a biophysicist applying advanced quantitative microscopy to study cell biology and single molecules. In my research I develop and adapt new microsocopy techniques as well as the appropriate labeling strategies to adress specific problems in (neuro-)biology, (bio)chemistry and the material sciences using molecular scale readout. I collaborate with biologists, chemists, engineers and physicists to identify new strategies to complement traditional measurement tools. My multidisciplinary background is based on a general physics education with specialization in biophysics and a doctorate in an interdisciplinary single molecule spectroscopy group.

Skills and Competences

Optics/Physical Chemistry
Light and fluorescence microsopy, Super-resolution Microscopy, fluorophore photophysics, ensemble and single-molecule fluorescence spectroscopy including fluorescence anisotropy and fluorescence lifetime measurements, Alternating Laser-excitation FRET, fluorophore/protein quantification methods (amongst others photon antibunching, photobleaching step analysis), single-molecule kinetics
protein preparation and purification, surface passivation and immobilization, fluorescence labeling of proteins and DNA, DNA thermodynamics, DNA biosensors and transcription factors, PAGE and EMSA
Computational skills
Mac and PC systems, Matlab, Latex, Monte Carlo Simulation


  • 03/2012 – 10/2015

    doctorate in physics, Heidelberg University, Germany
    dissertation on Fluorescence Quantification by Photon Statistics: From Objective Characterization to Application under supervision of Prof. D.–P. Herten
  • 10/2005 – 11/2011

    diploma in physics, Heidelberg University, Germany
    specialization in biophysics
    diploma thesis on DNA Biosensors for Transcription Factor Detection with Single Molecule Fluorescence Spectroscopy under supervision of PD D.–P. Herten and of Prof. W. Petrich
  • 08/2008 – 05/2009

    physics graduate program, Cornell University, Ithaca, NY, U.S.A.
    exchange student

Professional experience

  • 04/2017 – present

    Marie Sklodowska-Curie Individual Fellowship
    École Polytechnique Fédérale de Lausanne, Switzerland
    Advisors: Prof. T. Lasser, Prof. A. Radenovic and Prof. H. Lashuel
    Quantitative super-resolution optical fluctuation and phase microscopy for structural and functional imaging of Parkinson's disease
  • 07/2016 – 03/2017

    postdoctoral research assistant
    École Polytechnique Fédérale de Lausanne, Switzerland
    development of advanced imaging techniques (super-resolution optical fluctuation imaging, phase tomography) in a collaboration of the Laboratoire d’Optique Biomedicale of Prof. T. Lasser with the Medical Image Processing Lab of Prof. D. Van de Ville and the Laboratory of Nanoscale Biology of Prof. A. Radenovic
  • 12/2011 – 03/2016

    research assistant/fellow of DFG graduate college 1114
    Institute for Physical Chemistry and Center for Advanced Materials, Heidelberg University, Germany
    quantitative fluorescence microscopy in the Single Molecule Spectroscopy group of Prof. D.–P. Herten
  • 09/2010 – 11/2011

    student research assistant
    CellNetworks Cluster of Excellence, Heidelberg University, Heidelberg, Germany
    preparation of lecture and seminar course material for Prof. D.–P. Herten
  • 01/2009 – 05/2009

    project in experimental physics
    Department of Physics, Cornell University, Ithaca, NY, U.S.A.
    surface passivation of sample chambers for single molecule optical trapping in the HHMI single molecule biophysics laboratory of Prof. M. Wang
  • 09/2007 – 07/2008

    student research assistant, internship
    Department of Biomolecular Mechanisms (Prof. I. Schlichting), Max-Planck-Institute for Medical Research Heidelberg, Germany
    automatisation of MALDI-TOF mass spectrometry analysis of macromolecules, biophysics and molecular biology techniques

Prices and fellowships

  • 04/2017 – present

    Marie Sklodowska-Curie Individual Fellowship

  • 12/2011 – 12/2014

    fellow of DFG graduate college 1114 Optical Techniques for the Characterization of Interfaces

  • 08/2008 – 05/2009

    Cornell-Heidelberg Scholarship Exchange Student, Cornell University, Ithaca, NY, U.S.A.

  • 08/2008 – 05/2009

    Fulbright Travel Grant with participation in the Fulbright Scholarship Program


  • March 2018

    Winter School on "Fluorescence markers for advanced microscopy From photophysics to biology"
    Ecole de Physique des Houches, France
    Lecture on "Quantitative Aspects of Single Molecule Localization Microscopy". This biophysics school aimed at training students and researchers to master fluorescent markers used in advanced fluorescence bioimaging.
  • January 2017

    10th HGSFP Winterschool 2017
    University Center Obergurgl, Austria
    Two lectures on "Single-Molecule Fluorescence and Super-Resolution Imaging" for students from all branches of the Heidelberg Graduate School of Fundamental Physics.
  • 2012 – 2016

    supervision of master students
    Heidelberg University, Germany
    6-8 weeks practical students and master projects in the Single Molecule Spectroscopy group of Prof. D.–P. Herten
  • 2011 – 2015

    teaching assistant
    Heidelberg University, Germany
    conception and realization of 1 week practical course as part of Quantitative Biology in the master program for systems biology, preparation of course material and supervision of seminar lectures in Single Molecule Spectroscopy with Prof. D.–P. Herten
  • 10/2012 – 02/2013

    teaching assistant
    Heidelberg University, Germany
    Experimental Optics and Photonics with Prof. W. Petrich
  • 2005 - now

    skiing instructor (DSV Basic-Instructor for SZ Bad Herrenalb, Bad Herrenalb, Germany)